JMD etoc alert
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Material
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ventura, R. A.
Right arrow Articles by Siebert, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ventura, R. A.
Right arrow Articles by Siebert, R.
JMD 2006, Vol. 8, No. 2
Copyright © 2006 American Society for Investigative Pathology & Association for Molecular Pathology

FISH Analysis for the Detection of Lymphoma-Associated Chromosomal Abnormalities in Routine Paraffin-Embedded Tissue

Roland A. Ventura*, Jose I. Martin-Subero{dagger}, Margaret Jones*, Joanna McParland{ddagger}, Stefan Gesk{dagger}, David Y. Mason* and Reiner Siebert{dagger}

From the LRF Immunodiagnostics Unit, * Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford, United Kingdom; the Institute of Human Genetics, {dagger} University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany; and the Cytogenetics Department, {ddagger} Churchill Hospital, Oxford, United Kingdom

Over the last decade, fluorescence in situ hybridization (FISH) has become a firmly established technique in the diagnosis and assessment of lymphoid malignancies. However, this technique is not wide-ly used in the routine diagnostic evaluation of paraffin-embedded biopsies, most likely because of a perception that it is technically more demanding. There are also uncertainties regarding diagnostic thresholds and the way in which results should be interpreted. In this Review, we describe practical strategies for using FISH analysis to detect lymphoma-associated chromosomal abnormalities in routine paraffin-embedded lymphoma biopsies. Furthermore, we provide proposals on how FISH results should be interpreted (including how to calculate cutoff levels for FISH probes), recorded, and reported. An online appendix (available at http://jmd.amjpathol.org) details various simple, yet robust procedures for paraffin FISH analysis; it also provides additional information on the production of FISH probes, evaluating and reporting FISH results, sources for reagents and equipment, and troubleshooting. We hope that these suggestions will make FISH technology for the study of lymphoma biopsies more accessible to routine diagnostic and research laboratories.




This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
B. Sadikovic, M. Yoshimoto, S. Chilton-MacNeill, P. Thorner, J. A. Squire, and M. Zielenska
Identification of interactive networks of gene expression associated with osteosarcoma oncogenesis by integrated molecular profiling
Hum. Mol. Genet., June 1, 2009; 18(11): 1962 - 1975.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
H. W. Mensink, J. Vaarwater, E. Kilic, N. C. Naus, N. Mooy, G. Luyten, H. T. Bruggenwirth, D. Paridaens, and A. de Klein
Chromosome 3 Intratumor Heterogeneity in Uveal Melanoma
Invest. Ophthalmol. Vis. Sci., February 1, 2009; 50(2): 500 - 504.
[Abstract] [Full Text] [PDF]

Home page
 Am J Clin PatholHome page
G. R. de Paiva, J. Vassallo, and P. Brousset
The Authors' Reply
Am J Clin Pathol, January 1, 2009; 131(1): 145 - 146.
[Full Text] [PDF]

Home page
 haematolHome page
S. Bottcher, M. Ritgen, S. Buske, S. Gesk, W. Klapper, E. Hoster, W. Hiddemann, M. Unterhalt, M. Dreyling, R. Siebert, et al.
Minimal residual disease detection in mantle cell lymphoma: methods and significance of four-color flow cytometry compared to consensus IGH-polymerase chain reaction at initial staging and for follow-up examinations
Haematologica, April 1, 2008; 93(4): 551 - 559.
[Abstract] [Full Text] [PDF]

Home page
 Arch DermatolHome page
K. M. Kaune, M. Baumgart, S. Gesk, C. Mitteldorf, J. Baesecke, B. Glass, D. Haase, R. Siebert, B. M. Ghadimi, C. Neumann, et al.
Bullous Sweet Syndrome in a Patient With t(9;22)(q34;q11)-Positive Chronic Myeloid Leukemia Treated With the Tyrosine Kinase Inhibitor Nilotinib: Interphase Cytogenetic Detection of BCR-ABL- Positive Lesional Cells
Arch Dermatol, March 1, 2008; 144(3): 361 - 364.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
S. Ogino and A. Goel
Molecular Classification and Correlates in Colorectal Cancer
J. Mol. Diagn., January 1, 2008; 10(1): 13 - 27.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
G. Mattsson, S. Y. Tan, D. J.P. Ferguson, W. Erber, S. H. Turner, T. Marafioti, and D. Y. Mason
Detection of Genetic Alterations by ImmunoFISH Analysis of Whole Cells Extracted from Routine Biopsy Material
J. Mol. Diagn., September 1, 2007; 9(4): 479 - 489.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
N. Masir, R. Ventura, M. Jones, T. Marafioti, D. Y Mason, and J. Samol
Follicular lymphoma with trisomy 18 exhibiting loss of BCL-2 expression on transformation to a large cell lymphoma
J. Clin. Pathol., September 1, 2007; 60(9): 1061 - 1064.
[Full Text] [PDF]

Home page
 NEJMHome page
M. Hummel, S. Bentink, H. Berger, W. Klapper, S. Wessendorf, T. F.E. Barth, H.-W. Bernd, S. B. Cogliatti, J. Dierlamm, A. C. Feller, et al.
A biologic definition of Burkitt's lymphoma from transcriptional and genomic profiling.
N. Engl. J. Med., June 8, 2006; 354(23): 2419 - 2430.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the American Society for Investigative Pathology and the Association for Molecular Pathology.