JMD TIDES - Oligonucleotide and Peptide - May 18-21, 2008, Las Vegas, NV
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JMD 2005, Vol. 7, No. 5
Copyright © 2005 American Society for Investigative Pathology & Association for Molecular Pathology

A Practical Approach to the Detection of Prognostically Significant Genomic Aberrations in Multiple Myeloma

Zhong Chen*, Bonnie Issa*, Shiang Huang{dagger}, Emily Aston*, Jia Xu*, Margaret Yu{ddagger}, Arthur R. Brothman* and Martha Glenn{ddagger}

From the Division of Medical Genetics, * Department of Pediatrics and Affiliated Regional University Pathology Laboratories, Division of Clinical Oncology and the Lymphoproliferative Disease Group, {ddagger} Department of Internal Medicine, University of Utah School of Medicine, Salt Lake City, Utah; and the Center for Stem Cell Research and Application, {dagger} Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, People’s Republic of China

Multiple myeloma (MM) is a malignancy of differentiated B lymphocytes and has remained an incurable disease. Chromosomal abnormalities are among the most important prognostic parameters for MM. Cytoplasm immunoglobulin-enhanced interphase fluorescent in situ hybridization (FISH) has been a standard cell-targeting method for identifying genomic aberrations in MM. We have developed another cell-targeting approach by using CD138 magnetic microbeads to sort plasma cells for FISH analysis. The FISH panel consisted of four probes targeting RB-1, D13S319, immunoglobulin H, and p53 loci. We reviewed the FISH and conventional cytogenetic results of 60 patients with MM. The present cell-targeting approach in conjunction with the FISH probe panel was more sensitive than FISH performed on untargeted cells in detecting prognostically significant genomic aberrations (72 versus 24%, P = 0.0016). The frequencies of genomic abnormalities identified were similar to previously reported data obtained with the standard cell-targeting method. Therefore, our cell-targeting approach and FISH panel reliably detect prognostically important genomic abnormalities in MM and are potentially suitable for widespread use.




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Fluorescence in Situ Hybridization Analysis of Immunoglobulin Heavy Chain Translocations in Plasma Cell Myeloma Using Intact Paraffin Sections and Simultaneous CD138 Immunofluorescence
J. Mol. Diagn., September 1, 2006; 8(4): 459 - 465.
[Abstract] [Full Text] [PDF]


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