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JMD 2005, Vol. 7, No. 4
Copyright © 2005 American Society for Investigative Pathology & Association for Molecular Pathology

Microsatellite Analysis of Pleural Supernatants Could Increase Sensitivity of Pleural Fluid Cytology

Matthias Woenckhaus^*, Ulrike Grepmeier^*, Bernhard Werner, Christian Schulz, Felix Rockmann, Peter J. Wild^*, Georg Röckelein, Hagen Blaszyk^¶, Marion Schuierer^*, Ferdinand Hofstaedter^*, Arndt Hartmann^* and Wolfgang Dietmaier^*

From the Departments of Pathology * and Internal Medicine, University of Regensburg, Germany; the Department of Internal Medicine, Hospital Donaustauf, Donaustauf, Germany; the Pathology Institute, Regensburg, Germany; and the Department of Pathology, ^¶ University of Vermont College of Medicine, Burlington, Vermont

Pleural effusions may result from various inflammatory, hemodynamic, or neoplastic conditions. A common diagnostic problem lies in distinguishing malignant from benign pleural effusions using routine cytological evaluation. We studied pleural fluid samples obtained from 14 patients with histologically confirmed malignancy and from 6 patients with benign pleural effusions using 12 microsatellite markers from 8 different chromosomal regions. Supernatants and cellular sediments of all 20 pleural fluid samples were analyzed. Routine cytological examination was 100% specific for malignancy but was only 57% sensitive. Microsatellite analyses of pleural fluid supernatants showed genetic alterations in tumor patients only. However, 50% of pleural effusions that were considered negative for malignancy by routine cytological analysis showed either loss of heterozygosity or microsatellite instability. The sensitivity of pleural fluid examination rose to 79% when routine cytological assessment was supplemented by molecular studies. Our data suggest that microsatellite analysis increases the sensitivity of cytological pleural fluid examination in assessing potential malignancy and that combining cytological and molecular methods may improve yield and certainty in diagnostically challenging cases.

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