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JMD 2005, Vol. 7, No. 2
Copyright © 2005 American Society for Investigative Pathology & Association for Molecular Pathology

Molecular Typing of West Nile Virus, Dengue, and St. Louis Encephalitis Using Multiplex Sequencing

Thuraiayah Vinayagamoorthy*, Kirk Mulatz*, Michael Drebot{dagger} and Roger Hodkinson*

From Bio-ID Diagnostic Inc., * Saskatoon, Saskatchewan; and the National Microbiology Laboratory, {dagger} Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada

We report the development of an assay to simultaneously identify three of the clinically important flaviviruses (West Nile Virus, Dengue, and St. Louis encephalitis). This assay is based on the nucleotide sequence variations within a 266-bp region of the non-structural protein 5. Further, based on the nucleotide variations in the same region of the non-structural protein 5, four of the present Dengue serotypes were identified. To identify some of the subtypes of WNV we have developed a second assay using multiplex sequencing technology. The format of the result of this assay is an electropherogram of two genomic segments of the WNV genome: a 48-nucleotide sequence from the anchored core protein C and a 45-nucleotide sequence coding for the non-structural proteins (proteinase and putative helicase genes).






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Copyright © 2005 by the American Society for Investigative Pathology and the Association for Molecular Pathology.