| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
From the Laboratory of Diagnostic Molecular Oncology,
*
Division of Anatomic Pathology, University of Massachusetts Medical School, Worcester, Massachusetts; and the Laboratory of Diagnostic Molecular Hematology/Oncology,
Department of Pathology and Laboratory Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts
Human papillomavirus (HPV) is the worldwide cause of carcinoma of the uterine cervix, a cancer that is the second most common neoplasm in women, resulting in nearly 250,000 deaths a year. The magnitude of the risk of cancer after HPV infection, however, is virus type-specific. Over 40 HPV types can infect the genital tract. Comprehensive, high-throughput typing assays for HPV, however, are not currently available. Blending multiplex PCR and multiplex hybridization using spectrally addressable liquid bead microarrays we have developed a high-throughput, fast, single-tube-typing assay capable of simultaneously typing 45 HPV. The overall incidence of HPV in 429 women tested using this new assay was 72.2% for those with squamous intraepithelial lesions, 51.5% for those with atypical squamous cells of undetermined significance and 15.4% for women with normal cytology, respectively. This compared well with the incidence of HPV detected by a parallel non-typing generic high-risk assay. The new assay detected a wide spectrum of HPV types and a high incidence of mixed infections. We believe our assay may find widespread applications in areas requiring virus type-specific information, such as in epidemiological studies, cancer screening programs, monitoring therapeutic interventions, and evaluating the efficacy of HPV vaccine trials.
This article has been cited by other articles:
 |
|
 |
|
  J.-j. Choi, C. Kim, and H. Park Peptide Nucleic Acid-Based Array for Detecting and Genotyping Human Papillomaviruses J. Clin. Microbiol., June 1, 2009; 47(6): 1785 - 1790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Feng, S. Cherne, R. L. Winer, A. Balasubramanian, S.-K. Lee, S. E. Hawes, N. B. Kiviat, and L. A. Koutsky Development and Evaluation of a Liquid Bead Microarray Assay for Genotyping Genital Human Papillomaviruses J. Clin. Microbiol., March 1, 2009; 47(3): 547 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-L. Jiang, H.-H. Zhu, L.-F. Zhou, F. Chen, and Z. Chen Genotyping of human papillomavirus in cervical lesions by L1 consensus PCR and the Luminex xMAP system J. Med. Microbiol., June 1, 2006; 55(6): 715 - 720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Schmitt, I. G. Bravo, P. J. F. Snijders, L. Gissmann, M. Pawlita, and T. Waterboer Bead-Based Multiplex Genotyping of Human Papillomaviruses J. Clin. Microbiol., February 1, 2006; 44(2): 504 - 512. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
