JMD ASIP MEMBERSHIP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Warner, K. A.
Right arrow Articles by Willey, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Warner, K. A.
Right arrow Articles by Willey, J. C.
JMD 2003, Vol. 5, No. 3
Copyright © 2003 American Society for Investigative Pathology & Association for Molecular Pathology

The c-myc x E2F-1/p21 Interactive Gene Expression Index Augments Cytomorphologic Diagnosis of Lung Cancer in Fine-Needle Aspirate Specimens

Kristy A. Warner*, Erin L. Crawford*, Aiman Zaher{dagger}, Robert J. Coombs{ddagger}, Haitham Elsamaloty{ddagger}, Stacie L. Roshong-Denk{dagger}, Imran Sharief*, Guillermo V. Amurao*, Yongsook Yoon*, Amro Y. Al-Astal*, Ragheb A. Assaly*, Dawn-Alita R. Hernandez*, Timothy G. Graves*, Charles R. Knight*, Michael W. Harr*, Todd B. Sheridan§, Jeffrey P. DeMuth*, Robert J. Zahorchak, Jeffrey R. Hammersley*, Dan E. Olson*, Samuel J. Durham* and James C. Willey*{dagger}

From the Departments of Medicine, * Pathology, {dagger} and Radiology, {dagger} Medical College of Ohio, Toledo, Ohio; Gene Express, Inc., Toledo, Ohio; and the Department of Pathology, § Johns Hopkins University, Baltimore, Maryland

Morphological analysis of cytologic samples obtained by fine-needle aspirate (FNA) or bronchoscopy is an important method for diagnosing bronchogenic carcinoma. However, this approach has only about 65 to 80% diagnostic sensitivity. Based on previous studies, the c-myc x E2F-1/p21WAF1/CIP1 (p21 hereafter) gene expression index is highly sensitive and specific for distinguishing normal from malignant bronchial epithelial tissues. In an effort to improve sensitivity of diagnosing lung cancer in cytologic specimens, we used Standardized Reverse Transcriptase Polymerase Chain Reaction (StaRT-PCR) to measure the c-myc x E2F-1/p21 index in cDNA samples from 14 normal lung samples (6 normal lung parenchyma and 8 normal bronchial epithelial cell [NBEC] biopsies), and 16 FNA biopsies from 14 suspected tumors. Based on cytomorphologic criteria, 11 of the 14 suspected tumors were diagnosed as bronchogenic carcinoma and three specimens were non-diagnostic. Subsequent biopsy samples confirmed that the three non-diagnostic samples were derived from lung carcinomas. The index value for each bronchogenic carcinoma was above a cut-off value of 7000 and the index value of all but one normal sample was below 7000. Thus the c-myc x E2F-1/p21 index may augment cytomorphologic diagnosis of bronchogenic carcinoma biopsy samples, particularly those considered non-diagnostic by cytomorphologic criteria.




This article has been cited by other articles:


Home page
 Clin. Chem.Home page
E. H. Peters, S. Rojas-Caro, M. G. Brigell, R. J. Zahorchak, S. A. des Etages, P. L. Ruppel, C. R. Knight, B. Austermiller, M. C. Graham, S. Wowk, et al.
Quality-Controlled Measurement Methods for Quantification of Variations in Transcript Abundance in Whole Blood Samples from Healthy Volunteers
Clin. Chem., June 1, 2007; 53(6): 1030 - 1037.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the American Society for Investigative Pathology and the Association for Molecular Pathology.