JMD ASIP MEMBERSHIP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Godfrey, T. E.
Right arrow Articles by Jensen, R. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Godfrey, T. E.
Right arrow Articles by Jensen, R. H.
JMD 2000, Vol. 2, No. 2
Copyright © 2000 American Society for Investigative Pathology & Association for Molecular Pathology

Quantitative mRNA Expression Analysis from Formalin-Fixed, Paraffin-Embedded Tissues Using 5' Nuclease Quantitative Reverse Transcription-Polymerase Chain Reaction

Tony E. Godfrey*, Sun-Hun Kim§, Marielena Chavira*, David W. Ruff{ddagger}, Robert S. Warren{dagger}, Joe W. Gray* and Ronald H. Jensen*

From the Departments of Laboratory Medicine * and Surgery, {dagger} University of California San Francisco Cancer Center, San Francisco, California; P.E. Biosystems, {ddagger} Foster City, California; and the School of Dentistry, § Chonnam National University, Kwangju, South Korea

Analysis of gene expression and correlation with clinical parameters has the potential to become an important factor in therapeutic decision making. The ability to analyze gene expression in archived tissues, for which clinical followup is already available, will greatly facilitate research in this area. A major obstacle to this approach, however, has been the uncertainty about whether gene expression analyses from routinely archived tissues accurately reflect expression before fixation. In the present study we have optimized the RNA isolation and reverse transcription steps for quantitative reverse transcription-polymerase chain reaction (RT-PCR) on archival material. Using tissue taken directly from the operating room, mRNAs with half-lives from 10 minutes to >8 hours were isolated and reverse transcribed. Subsequent real-time quantitative PCR methodology (TaqMan) on these cDNAs gives a measurement of gene expression in the fixed tissues comparable to that in the fresh tissue. In addition, we simulated routine pathology handling and demonstrate that this method of mRNA quantitation is insensitive to pre-fixation times (time from excision to fixation) of up to 12 hours. Therefore, it should be feasible to analyze gene expression in archived tissues where tissue collection procedures are largely unknown.




This article has been cited by other articles:


Home page
 J. Clin. Pathol.Home page
U Siebolts, H Varnholt, U Drebber, H-P Dienes, C Wickenhauser, and M Odenthal
Tissues from routine pathology archives are suitable for microRNA analyses by quantitative PCR
J. Clin. Pathol., January 1, 2009; 62(1): 84 - 88.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
K. J. Wiggins, R. M. Gow, J. Kanellis, P. Hill, D. J. Kelly, A. Skene, D. J. Goodman, and R. G. Langham
BK virus RNA can be detected in archival renal transplant biopsies using the reverse trancription polymerase chain reaction
Nephrol. Dial. Transplant., December 4, 2008; (2008) gfn656v1.
[Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
J.-Y. Chung, T. Braunschweig, R. Williams, N. Guerrero, K. M. Hoffmann, M. Kwon, Y. K. Song, S. K. Libutti, and S. M. Hewitt
Factors in Tissue Handling and Processing That Impact RNA Obtained From Formalin-fixed, Paraffin-embedded Tissue
J. Histochem. Cytochem., November 1, 2008; 56(11): 1033 - 1042.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. Xi, A. Feber, V. Gupta, M. Wu, A. D. Bergemann, R. J. Landreneau, V. R. Litle, A. Pennathur, J. D. Luketich, and T. E. Godfrey
Whole genome exon arrays identify differential expression of alternatively spliced, cancer-related genes in lung cancer
Nucleic Acids Res., November 1, 2008; 36(20): 6535 - 6547.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. E. Szafranska, M. Doleshal, H. S. Edmunds, S. Gordon, J. Luttges, J. B. Munding, R. J. Barth Jr, E. J. Gutmann, A. A. Suriawinata, J. Marc Pipas, et al.
Analysis of MicroRNAs in Pancreatic Fine-Needle Aspirates Can Classify Benign and Malignant Tissues
Clin. Chem., October 1, 2008; 54(10): 1716 - 1724.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
G. R. Varadhachary, D. Talantov, M. N. Raber, C. Meng, K. R. Hess, T. Jatkoe, R. Lenzi, D. R. Spigel, Y. Wang, F. A. Greco, et al.
Molecular Profiling of Carcinoma of Unknown Primary and Correlation With Clinical Evaluation
J. Clin. Oncol., September 20, 2008; 26(27): 4442 - 4448.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
A. E. Szafranska, T. S. Davison, J. Shingara, M. Doleshal, J. A. Riggenbach, C. D. Morrison, S. Jewell, and E. Labourier
Accurate Molecular Characterization of Formalin-Fixed, Paraffin-Embedded Tissues by microRNA Expression Profiling
J. Mol. Diagn., September 1, 2008; 10(5): 415 - 423.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. Rappocciolo, H. R. Hensler, M. Jais, T. A. Reinhart, A. Pegu, F. J. Jenkins, and C. R. Rinaldo
Human Herpesvirus 8 Infects and Replicates in Primary Cultures of Activated B Lymphocytes through DC-SIGN
J. Virol., May 15, 2008; 82(10): 4793 - 4806.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
M. Doleshal, A. A. Magotra, B. Choudhury, B. D. Cannon, E. Labourier, and A. E. Szafranska
Evaluation and Validation of Total RNA Extraction Methods for MicroRNA Expression Analyses in Formalin-Fixed, Paraffin-Embedded Tissues
J. Mol. Diagn., May 1, 2008; 10(3): 203 - 211.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Qin, Y. Sui, A. C. Soloff, B. A. Fallert Junecko, D. E. Kirschner, M. A. Murphey-Corb, S. C. Watkins, P. M. Tarwater, J. E. Pease, S. M. Barratt-Boyes, et al.
Chemokine and Cytokine Mediated Loss of Regulatory T Cells in Lymph Nodes during Pathogenic Simian Immunodeficiency Virus Infection
J. Immunol., April 15, 2008; 180(8): 5530 - 5536.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
B. S. Knudsen, A. N. Allen, D. F. McLerran, R. L. Vessella, J. Karademos, J. E. Davies, B. Maqsodi, G. K. McMaster, and A. R. Kristal
Evaluation of the Branched-Chain DNA Assay for Measurement of RNA in Formalin-Fixed Tissues
J. Mol. Diagn., March 1, 2008; 10(2): 169 - 176.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
K. Steger, J. Wilhelm, L. Konrad, T. Stalf, R. Greb, T. Diemer, S. Kliesch, M. Bergmann, and W. Weidner
Both protamine-1 to protamine-2 mRNA ratio and Bcl2 mRNA content in testicular spermatids and ejaculated spermatozoa discriminate between fertile and infertile men
Hum. Reprod., January 1, 2008; 23(1): 11 - 16.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
T. Haque, D. Faury, S. Albrecht, E. Lopez-Aguilar, P. Hauser, M. Garami, Z. Hanzely, L. Bognar, R. F. Del Maestro, J. Atkinson, et al.
Gene Expression Profiling from Formalin-Fixed Paraffin-Embedded Tumors of Pediatric Glioblastoma
Clin. Cancer Res., November 1, 2007; 13(21): 6284 - 6292.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
L. Xi, D. G. Nicastri, T. El-Hefnawy, S. J. Hughes, J. D. Luketich, and T. E. Godfrey
Optimal Markers for Real-Time Quantitative Reverse Transcription PCR Detection of Circulating Tumor Cells from Melanoma, Breast, Colon, Esophageal, Head and Neck, and Lung Cancers
Clin. Chem., July 1, 2007; 53(7): 1206 - 1215.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C.-J. Li, W. Ning, M. A. Matthay, C. A. Feghali-Bostwick, and A. M. K. Choi
MAPK pathway mediates EGR-1-HSP70-dependent cigarette smoke-induced chemokine production
Am J Physiol Lung Cell Mol Physiol, May 1, 2007; 292(5): L1297 - L1303.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
W. Ning, Y. Dong, J. Sun, C. Li, M. A. Matthay, C. A. Feghali-Bostwick, and A. M. K. Choi
Cigarette Smoke Stimulates Matrix Metalloproteinase-2 Activity via EGR-1 in Human Lung Fibroblasts
Am. J. Respir. Cell Mol. Biol., April 1, 2007; 36(4): 480 - 490.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
R. A. Coudry, S. I. Meireles, R. Stoyanova, H. S. Cooper, A. Carpino, X. Wang, P. F. Engstrom, and M. L. Clapper
Successful Application of Microarray Technology to Microdissected Formalin-Fixed, Paraffin-Embedded Tissue
J. Mol. Diagn., February 1, 2007; 9(1): 70 - 79.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. A. de Boer, E. S. Jordanova, G. G. Kenter, A. A. Peters, W. E. Corver, J. B. Trimbos, and G. J. Fleuren
High Human Papillomavirus Oncogene mRNA Expression and Not Viral DNA Load Is Associated with Poor Prognosis in Cervical Cancer Patients
Clin. Cancer Res., January 1, 2007; 13(1): 132 - 138.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
X. M. Wang, Y. Zhang, H. P. Kim, Z. Zhou, C. A. Feghali-Bostwick, F. Liu, E. Ifedigbo, X. Xu, T. D. Oury, N. Kaminski, et al.
Caveolin-1: a critical regulator of lung fibrosis in idiopathic pulmonary fibrosis
J. Exp. Med., December 25, 2006; 203(13): 2895 - 2906.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
S. Uccella, R. Cerutti, D. Vigetti, D. Furlan, R. Oldrini, I. Carnevali, G. Pelosi, S. La Rosa, A. Passi, and C. Capella
Histidine Decarboxylase, DOPA Decarboxylase, and Vesicular Monoamine Transporter 2 Expression in Neuroendocrine Tumors: Immunohistochemical Study and Gene Expression Analysis
J. Histochem. Cytochem., August 1, 2006; 54(8): 863 - 875.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Surg. Oncol.Home page
S. Wada, T. Noguchi, S. Takeno, and K. Kawahara
PIK3CA and TFRC Located in 3q Are New Prognostic Factors in Esophageal Squamous Cell Carcinoma
Ann. Surg. Oncol., July 1, 2006; 13(7): 961 - 966.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
D. Talantov, J. Baden, T. Jatkoe, K. Hahn, J. Yu, Y. Rajpurohit, Y. Jiang, C. Choi, J. S. Ross, D. Atkins, et al.
A Quantitative Reverse Transcriptase-Polymerase Chain Reaction Assay to Identify Metastatic Carcinoma Tissue of Origin
J. Mol. Diagn., July 1, 2006; 8(3): 320 - 329.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
A Khalid, S Finkelstein, B Thompson, L Kelly, C Hanck, T E Godfrey, and D C Whitcomb
A 93 year old man with the PRSS1 R122H mutation, low SPINK1 expression, and no pancreatitis: insights into phenotypic non-penetrance
Gut, May 1, 2006; 55(5): 728 - 731.
[Abstract] [Full Text] [PDF]

Home page
 CSH ProtocolsHome page
M. A. Connolly, P. A. Clausen, and J. G. Lazar
Preparation of RNA from Paraffin-Embedded Fixed Tissue Using Trizol
CSH Protocols, May 1, 2006; 2006(1): pdb.prot4103 - pdb.prot4103.
[Full Text]

Home page
 Clin. Cancer Res.Home page
L. Xi, M. C. Coello, V. R. Litle, S. Raja, W. E. Gooding, S. A. Yousem, T. El-Hefnawy, R. J. Landreneau, J. D. Luketich, and T. E. Godfrey
A Combination of Molecular Markers Accurately Detects Lymph Node Metastasis in Non-Small Cell Lung Cancer Patients
Clin. Cancer Res., April 15, 2006; 12(8): 2484 - 2491.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
D. Chowdary, J. Lathrop, J. Skelton, K. Curtin, T. Briggs, Y. Zhang, J. Yu, Y. Wang, and A. Mazumder
Prognostic Gene Expression Signatures Can Be Measured in Tissues Collected in RNAlater Preservative
J. Mol. Diagn., February 1, 2006; 8(1): 31 - 39.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. W. Bartlett and M. E. Davis
Insights into the kinetics of siRNA-mediated gene silencing from live-cell and live-animal bioluminescent imaging
Nucleic Acids Res., January 12, 2006; 34(1): 322 - 333.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. A. Cobleigh, B. Tabesh, P. Bitterman, J. Baker, M. Cronin, M.-L. Liu, R. Borchik, J.-M. Mosquera, M. G. Walker, and S. Shak
Tumor Gene Expression and Prognosis in Breast Cancer Patients with 10 or More Positive Lymph Nodes
Clin. Cancer Res., December 15, 2005; 11(24): 8623 - 8631.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. M Schaefer, J. A. Wright, P. A. Pioli, and C. R. Wira
IL-1{beta}-Mediated Proinflammatory Responses Are Inhibited by Estradiol via Down-Regulation of IL-1 Receptor Type I in Uterine Epithelial Cells
J. Immunol., November 15, 2005; 175(10): 6509 - 6516.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
R. Langer, K. Specht, K. Becker, P. Ewald, M. Bekesch, M. Sarbia, R. Busch, M. Feith, H. J. Stein, J.-R. Siewert, et al.
Association of Pretherapeutic Expression of Chemotherapy-Related Genes with Response to Neoadjuvant Chemotherapy in Barrett Carcinoma
Clin. Cancer Res., October 15, 2005; 11(20): 7462 - 7469.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
S. Raja, J. Ching, L. Xi, S. J. Hughes, R. Chang, W. Wong, W. McMillan, W. E. Gooding, K. S. McCarty Jr, M. Chestney, et al.
Technology for Automated, Rapid, and Quantitative PCR or Reverse Transcription-PCR Clinical Testing
Clin. Chem., May 1, 2005; 51(5): 882 - 890.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. L. Ferris, L. Xi, S. Raja, J. L. Hunt, J. Wang, W. E. Gooding, L. Kelly, J. Ching, J. D. Luketich, and T. E. Godfrey
Molecular Staging of Cervical Lymph Nodes in Squamous Cell Carcinoma of the Head and Neck
Cancer Res., March 15, 2005; 65(6): 2147 - 2156.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
L. Xi, J. D. Luketich, S. Raja, W. E. Gooding, V. R. Litle, M. C. Coello, S. D. Finkelstein, M. L. Chestney, R. J. Landreneau, S. J. Hughes, et al.
Molecular Staging of Lymph Nodes from Patients with Esophageal Adenocarcinoma
Clin. Cancer Res., February 1, 2005; 11(3): 1099 - 1109.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. M. Schaefer, J. V. Fahey, J. A. Wright, and C. R. Wira
Innate Immunity in the Human Female Reproductive Tract: Antiviral Response of Uterine Epithelial Cells to the TLR3 Agonist Poly(I:C)
J. Immunol., January 15, 2005; 174(2): 992 - 1002.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. Bibikova, J. M. Yeakley, E. Chudin, J. Chen, E. Wickham, J. Wang-Rodriguez, and J.-B. Fan
Gene Expression Profiles in Formalin-Fixed, Paraffin-Embedded Tissues Obtained with a Novel Assay for Microarray Analysis
Clin. Chem., December 1, 2004; 50(12): 2384 - 2386.
[Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
A. Gloghini, B. Canal, U. Klein, L. Dal Maso, T. Perin, R. Dalla-Favera, and A. Carbone
RT-PCR Analysis of RNA Extracted from Bouin-Fixed and Paraffin-Embedded Lymphoid Tissues
J. Mol. Diagn., November 1, 2004; 6(4): 290 - 296.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. Bibikova, D. Talantov, E. Chudin, J. M. Yeakley, J. Chen, D. Doucet, E. Wickham, D. Atkins, D. Barker, M. Chee, et al.
Quantitative Gene Expression Profiling in Formalin-Fixed, Paraffin-Embedded Tissues Using Universal Bead Arrays
Am. J. Pathol., November 1, 2004; 165(5): 1799 - 1807.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
W. Ning, C.-J. Li, N. Kaminski, C. A. Feghali-Bostwick, S. M. Alber, Y. P. Di, S. L. Otterbein, R. Song, S. Hayashi, Z. Zhou, et al.
Comprehensive gene expression profiles reveal pathways related to the pathogenesis of chronic obstructive pulmonary disease
PNAS, October 12, 2004; 101(41): 14895 - 14900.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
B. Sipos, W. Klapper, M.-L. Kruse, H. Kalthoff, D. Kerjaschki, and G. Kloppel
Expression of Lymphangiogenic Factors and Evidence of Intratumoral Lymphangiogenesis in Pancreatic Endocrine Tumors
Am. J. Pathol., October 1, 2004; 165(4): 1187 - 1197.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
R. C. K. Jordan, M. Macabeo-Ong, C. H. Shiboski, N. Dekker, D. G. Ginzinger, D. T. W. Wong, and B. L. Schmidt
Overexpression of Matrix Metalloproteinase-1 and -9 mRNA Is Associated with Progression of Oral Dysplasia to Cancer
Clin. Cancer Res., October 1, 2004; 10(19): 6460 - 6465.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Wang, L. Xi, J. L. Hunt, W. Gooding, T. L. Whiteside, Z. Chen, T. E. Godfrey, and R. L. Ferris
Expression Pattern of Chemokine Receptor 6 (CCR6) and CCR7 in Squamous Cell Carcinoma of the Head and Neck Identifies a Novel Metastatic Phenotype
Cancer Res., March 1, 2004; 64(5): 1861 - 1866.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
L. M. Gjerdrum, B. S. Sorensen, E. Kjeldsen, F. B. Sorensen, E. Nexo, and S. Hamilton-Dutoit
Real-Time Quantitative PCR of Microdissected Paraffin-Embedded Breast Carcinoma: An Alternative Method for HER-2/neu Analysis
J. Mol. Diagn., February 1, 2004; 6(1): 42 - 51.
[Abstract] [Full Text]

Home page
 Am. J. Pathol.Home page
M. Cronin, M. Pho, D. Dutta, J. C. Stephans, S. Shak, M. C. Kiefer, J. M. Esteban, and J. B. Baker
Measurement of Gene Expression in Archival Paraffin-Embedded Tissues: Development and Performance of a 92-Gene Reverse Transcriptase-Polymerase Chain Reaction Assay
Am. J. Pathol., January 1, 2004; 164(1): 35 - 42.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
K. Steger, L. Fink, K. Failing, R. M. Bohle, S. Kliesch, W. Weidner, and M. Bergmann
Decreased protamine-1 transcript levels in testes from infertile men
Mol. Hum. Reprod., June 1, 2003; 9(6): 331 - 336.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Anders, R. Hansen, R.-X. Ding, K. A. Rauen, M. J. Bissell, and W. M. Korn
Disruption of 3D tissue integrity facilitates adenovirus infection by deregulating the coxsackievirus and adenovirus receptor
PNAS, February 18, 2003; 100(4): 1943 - 1948.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
H. N. Abrahamsen, T. Steiniche, E. Nexo, S. J. Hamilton-Dutoit, and B. S. Sorensen
Towards Quantitative mRNA Analysis in Paraffin-Embedded Tissues Using Real-Time Reverse Transcriptase-Polymerase Chain Reaction: A Methodological Study on Lymph Nodes from Melanoma Patients
J. Mol. Diagn., February 1, 2003; 5(1): 34 - 41.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. Srinivasan, D. Sedmak, and S. Jewell
Effect of Fixatives and Tissue Processing on the Content and Integrity of Nucleic Acids
Am. J. Pathol., December 1, 2002; 161(6): 1961 - 1971.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X. Huang, S. M. Gollin, S. Raja, and T. E. Godfrey
High-resolution mapping of the 11q13 amplicon and identification of a gene, TAOS1, that is amplified and overexpressed in oral cancer cells
PNAS, August 20, 2002; 99(17): 11369 - 11374.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
S. Raja, T. El-Hefnawy, L. A. Kelly, M. L. Chestney, J. D. Luketich, and T. E. Godfrey
Temperature-controlled Primer Limit for Multiplexing of Rapid, Quantitative Reverse Transcription-PCR Assays: Application to Intraoperative Cancer Diagnostics
Clin. Chem., August 1, 2002; 48(8): 1329 - 1337.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
H. Kodama, T. Hirotani, Y. Suzuki, S. Ogawa, and K. Yamazaki
Cardiomyogenic Differentiation in Cardiac Myxoma Expressing Lineage-Specific Transcription Factors
Am. J. Pathol., August 1, 2002; 161(2): 381 - 389.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
L. Lamarcq, J. Deeds, D. Ginzinger, J. Perry, S. Padmanabha, and K. Smith-McCune
Measurements of Human Papillomavirus Transcripts by Real Time Quantitative Reverse Transcription-Polymerase Chain Reaction in Samples Collected for Cervical Cancer Screening
J. Mol. Diagn., May 1, 2002; 4(2): 97 - 102.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. A. Reinhart, B. A. Fallert, M. E. Pfeifer, S. Sanghavi, S. Capuano III, P. Rajakumar, M. Murphey-Corb, R. Day, C. L. Fuller, and T. M Schaefer
Increased expression of the inflammatory chemokine CXC chemokine ligand 9/monokine induced by interferon-gamma in lymphoid tissues of rhesus macaques during simian immunodeficiency virus infection and acquired immunodeficiency syndrome
Blood, May 1, 2002; 99(9): 3119 - 3128.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
S. Raja, J. D. Luketich, L. A. Kelly, W. E. Gooding, S. D. Finkelstein, and T. E. Godfrey
Rapid, quantitative reverse transcriptase-polymerase chain reaction: Application to intraoperative molecular detection of occult metastases in esophageal cancer
J. Thorac. Cardiovasc. Surg., March 1, 2002; 123(3): 475 - 483.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
D. G. Thomas, T. J. Giordano, D. Sanders, J. S. Biermann, and L. Baker
Absence of HER2/neu Gene Expression in Osteosarcoma and Skeletal Ewing's Sarcoma
Clin. Cancer Res., March 1, 2002; 8(3): 788 - 793.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
U. Lehmann, O. Bock, F. Langer, and H. Kreipe
Demonstration of Light Chain Restricted Clonal B-Lymphoid Infiltrates in Archival Bone Marrow Trephines by Quantitative Real-Time Polymerase Chain Reaction
Am. J. Pathol., December 1, 2001; 159(6): 2023 - 2029.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
T. E. Godfrey, S. Raja, S. D. Finkelstein, W. E. Gooding, L. A. Kelly, and J. D. Luketich
Prognostic Value of Quantitative Reverse Transcription-Polymerase Chain Reaction in Lymph Node-negative Esophageal Cancer Patients
Clin. Cancer Res., December 1, 2001; 7(12): 4041 - 4048.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the American Society for Investigative Pathology and the Association for Molecular Pathology.