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JMD 2000, Vol. 2, No. 1
Copyright © 2000 American Society for Investigative Pathology & Association for Molecular Pathology

Hepatosplenic and Subcutaneous Panniculitis-Like / T Cell Lymphomas Are Derived from Different V Subsets of / T Lymphocytes

Grzegorz K. Przybylski^*, Hong Wu, William R. Macon, Janet Finan, Debra G. B. Leonard, Raymond E. Felgar, Joseph A. DiGiuseppe, Peter C. Nowell, Steven H. Swerdlow^¶, Marshall E. Kadin^||, Mariusz A. Wasik and Kevin E. Salhany

From the Institute of Human Genetics, * Poznan, Poland; the University of Pennsylvania, Philadelphia, Pennsylvania; Vanderbilt University, Nashville, Tennessee; the Johns Hopkins University, Baltimore, Maryland; the University of Pittsburgh, ^¶ Pittsburgh, Pennsylvania; and Harvard University, ^|| Boston, Massachusetts

Gamma/delta T cell lymphomas (/ TCL) represent rare, often aggressive types of T cell malignancy that are clinically and pathologically diverse. Most / TCL occur as a hepatosplenic or subcutaneous type. To date, analysis of the T cell receptor (TCR) gene repertoire of hepatosplenic / TCL (/ HSTCL) and subcutaneous panniculitis-like / TCL (/ SPTCL) has been reported only in a limited number of cases. In this study we analyzed 11 / HSTCL and 4 / SPTCL by polymerase chain reaction and immunostaining to determine their usage of the V subtypes (V1–6). It is noteworthy that 10 of 11 / HSTCL expressed the V1 gene. The remaining case also expressed T cell receptor (TCR) as determined by flow cytometry and TCR rearrangement in Southern blot. However, the V gene expressed by this lymphoma could not be determined, which suggests usage of an as yet unidentified V gene. In striking contrast to the / HSTCL, all 4 / SPTCL expressed the V2 gene. Our data demonstrate that / HSTCL are preferentially derived from the V1 subset of / T lymphocytes, whereas / SPTCL are preferentially derived from the V2 subset. The pattern of V gene expression in HSTCL and SPTCL corresponds to the respective, predominant / T cell subsets normally found in the spleen and skin. This finding suggests that / TCL are derived from normal / T lymphocytes which reside in the affected tissues. Furthermore, the selective, lymphoma type-specific V gene segment usage may provide a molecular tool to distinguish better among various types of / TCL lymphoma particularly in the clinically advanced, widely disseminated cases.

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